Journal: Extracellular Vesicles and Circulating Nucleic Acids

Article Title: A single-sEV analysis identifies plasma EPCAM + sEVs as a biomarker for early diagnosis and monitoring postoperative remission of thyroid cancer

doi: 10.20517/evcna.2025.93

Figure Lengend Snippet: The function of HC-derived plasma sEVs in vitro . (A) Dio-labeled plasma sEVs from HC were taken up by TC cells whose nuclei were stained using DAPI; (B) CCK-8 analysis of TC cells treated with PBS or HC-derived plasma sEVs; Representative images of colony formation assays (C and D), migration assays (E and F), and invasion assays (G and H) of TC cells exposed to PBS or HC-derived plasma sEVs. The data was analyzed using the Student’s t -test. * P value < 0.05; ** P value < 0.01; *** P value < 0.001; ns: not significant. HC: Healthy controls; sEVs: small extracellular vesicles; TC: thyroid carcinoma; DAPI: 4′,6-diamidino-2-phenylindole; CCK-8: cell counting kit-8; PBS: phosphate-buffered saline; DIO: 3,3′-dioctadecyloxacarbocyanine perchlorate; OD450: optical density at 450 nm.

Article Snippet: The labeled sEVs were washed twice with RPMI-1640 medium to remove excess Dio, resuspended in PBS, and then co-cultured with TPC-1 or BCPAP cells at 37 °C for 24 h. After fixation with 4% paraformaldehyde, the cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining dye (Beyotime, China).

Techniques: Derivative Assay, Clinical Proteomics, In Vitro, Labeling, Staining, CCK-8 Assay, Migration, Cell Counting, Saline